phycoerythrin conjugated mouse anti chicken cd8a  (Bio-Rad)

Journal: Frontiers in Physiology

Article Title: Effects of Artemisia annua supplementation on the performance and gut health of laying hens challenged with mixed Eimeria species

doi: 10.3389/fphys.2024.1381548

Figure Lengend Snippet: Representative histogram of the gating protocol used on the enriched chicken peripheral blood lymphocytes stained with PE-CD4 and FITC CD-8. The cells were first gated based on the size (FSC-H) and granularity (SSC-H) (A) . The single cell population was then gated from the histogram (A) based on the cell area (SSC-A) and the cell height (SSC-H) (Fig. 1B). Singlet cells identified in (B) were then analyzed for PE-CD4 and FITC-CD8 staining (C) .

Article Snippet: In brief, 100 μL of the isolated cells were incubated with CD4 (mouse anti-chicken CD4-PE; SouthernBiotech, Birmingham, AL) and CD8 (mouse anti-chicken CD8a-FITC; SouthernBiotech, Birmingham, AL) antibodies.

Techniques: Staining

Journal: Frontiers in Physiology

Article Title: Effects of Artemisia annua supplementation on the performance and gut health of laying hens challenged with mixed Eimeria species

doi: 10.3389/fphys.2024.1381548

Figure Lengend Snippet: Effect of the inclusion of phytogenic feed additive A. annua in the diets of laying hens on their peripheral mononuclear blood cell count and CD4 + and CD8 + T cell population 14 days post- Eimeria challenge. At 25 weeks of age, 12,500 E. maxima , 12,500 E. maxima , and 62,500 E. tenella oocysts were inoculated in laying hens grouped into challenged control, 0.5% A. annua , and 1% A. annua . NC: non-challenged control; PF: pair-fed control; CC: challenged control (12,500 E. maxima ; 12,500 E. tenella ; and 62,500 E. acervulina oocysts per mL); 0.5AA: challenged control with the inclusion of 0.5% A. annua in the diet; 1AA: challenged control with the inclusion of 1% A. annua in the diet; dpi: days post-inoculation of Eimeria spp.

Article Snippet: In brief, 100 μL of the isolated cells were incubated with CD4 (mouse anti-chicken CD4-PE; SouthernBiotech, Birmingham, AL) and CD8 (mouse anti-chicken CD8a-FITC; SouthernBiotech, Birmingham, AL) antibodies.

Techniques: Cell Counting, Control

Journal: Poultry science

Article Title: Inhibitory effect of Eimeria maxima IFN-γ inhibitory molecules on the immune function of T cell subsets in chickens.

doi: 10.1016/j.psj.2023.103098

Figure Lengend Snippet: Figure 2. Detection of the sorting purity of chicken CD8+, CD4+, CD4+ CD25 and CD4+ CD25+ T cells by flow cytometry. (A) Magnetic bead sorting of chicken CD8+ T cells A1: blank cells (total PBMC); A2: CD8+ T cells before cell sorting; A3: CD8+ T cells after cell sorting. (B) Mag- netic bead sorting of chicken CD4+ T cells B1: blank cells (total PBMC); B2: CD4+ T cells before cell sorting; B3: CD4+ T cells after cell sorting. (C) Magnetic bead sorting of chicken CD4+ CD25 T cells C1: blank cells (total PBMC); C2: CD4+ CD25 T cells before cell sorting; C3: CD4+

Article Snippet: The primary phycoerythrin (PE)-conjugated mouse anti-chicken CD8a or CD4 antibody (Southernbiotech, Birmingham, AL) was added according to 1 £ 108 cells.

Techniques: Cytometry, FACS

Journal: Poultry science

Article Title: Inhibitory effect of Eimeria maxima IFN-γ inhibitory molecules on the immune function of T cell subsets in chickens.

doi: 10.1016/j.psj.2023.103098

Figure Lengend Snippet: Figure 5. Effects of rEmHPSP-2 and rEmHPSP-3 on the transcription of IFN-g and IL-2 in chicken CD8+ T cells (A) Effects of rEmHPSP-2 on the transcription of IFN-g and IL-2 in chicken CD8+ T cells. (B) Effects of rEmHPSP-3 on the transcription of IFN-g and IL-2 in chicken CD8+ T cells. The differences between groups were analyzed using Duncan method of 1-way ANOVA, where the same letter indicates no significant differ- ence (P > 0.05) and different letter indicates significant difference (P < 0.05).

Article Snippet: The primary phycoerythrin (PE)-conjugated mouse anti-chicken CD8a or CD4 antibody (Southernbiotech, Birmingham, AL) was added according to 1 £ 108 cells.

Techniques: